Experimental studies on male reproductive toxicity of bisphenol A in vitro and vivo.
- Author:
Mao-xian DENG
1
;
De-sheng WU
;
Xiang-gui CHEN
;
Li-shi ZHANG
;
Pei-yu XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Benzhydryl Compounds; Cells, Cultured; Cytoskeleton; drug effects; Male; Organ Size; drug effects; Phenols; toxicity; Rats; Rats, Sprague-Dawley; Sertoli Cells; cytology; drug effects; Testis; anatomy & histology; cytology; drug effects; Vimentin; metabolism
- From: Chinese Journal of Preventive Medicine 2004;38(6):383-387
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of Bisphenol A in adult rats and its possible mechanisms.
METHODSBPA (in corn oil) was administered orally to 9-week-old male Sprague-Dawley rats for 14 days (0, 1 and 5 g/kg bw), and incubated primary Sertoli cells from pubertal SD rats with 0, 10(-7), 10(-6), 10(-5), 10(-4) mol/L BPA.
RESULTSAfter oral administration, a significant decrease in right testis weight was observed in 5 g/kg dose group, but not in the 1 g/kg bw dose group. Germ cells were detached from basement membrane of seminiferous tubules and Sertoli cells in BPA-treated groups. Administration of BPA at 1 g/kg bw and 5 g/kg bw produced both nucleus pycnosis and vacuolized nucleus in germ cells and Sertoli cells. A marked loss in vimentin staining in Sertoli cells from testis of BPA-treated rats was detected. No change in levels of serum estradiol and testosterone was observed after two-week exposure to BPA. In Sertoli cell primary culture, BPA destroyed the cytoskeleton and cell-cell junctions, and elongated Sertoli cells.
CONCLUSIONThese results suggest that BPA may injure reproductive function of male rats by destroying the cytoskeleton and changing the form of Sertoli cells.
