Neuroprotection by resveratrol against cytotoxicity of oxidized low density lipoprotein in PC12 cells.
- Author:
Li CAO
1
;
Yong-jun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Survival; drug effects; Lipoproteins, LDL; toxicity; Neuroprotective Agents; pharmacology; PC12 Cells; Rats; Stilbenes; pharmacology
- From: Chinese Journal of Preventive Medicine 2005;39(1):19-21
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate neuroprotection of resveratrol against cytotoxicity of oxidized low density lipoprotein (oxLDL) in PC12 cells.
METHODSPC12 cells were pretreated with resveratrol for one hour as a neuron model and then exposed to oxLDL at varied concentrations. Effects of resveratrol on cell viability, permeability of cell membrane, cell nucleus and activity of caspase-3 were evaluated with MTT assay, lactic acid dehydrogenase (LDH) release assay, DNA fragmentation (TUNEL) assay and assay for caspase-3 activity (caspase assay).
RESULTSCell viability, LDH release rate, percentage of cells with positive TUNEL and activity of caspase-3 were (62 +/- 3)%, (23 +/- 3)%, (26 +/- 5)% and (0.811 +/- 0.049) mol.min(-1).microg(-1), vs (84 +/- 7)%, (13 +/- 4)%, (12 +/- 4)% and (0.553 +/- 0.048) mol.min(-1).microg(-1) in PC12 cells treated with 10 mg/L oxLDL vs in those treated with 10 mg/L oxLDL plus 50 micromol/L resveratrol, respectively, with statistically significant difference.
CONCLUSIONResveratrol could attenuate cytotoxicity induced by oxLDL in PC12 cells with neuroprotection.
