Effects of various iodin-nutritional on activity of T4 5'-and 5-deiodinase in rat brain.
- Author:
Shan-yi GUO
1
;
Ai-jun ZUO
;
Nian-qing LIU
;
Xue-qin ZHAO
;
Ruo-lin GUO
;
Fang ZHANG
;
Jing-yu ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; enzymology; Female; Iodide Peroxidase; metabolism; Iodine; administration & dosage; deficiency; Male; Rats; Rats, Wistar; Thyroxine; blood; Triiodothyronine; blood
- From: Chinese Journal of Preventive Medicine 2005;39(1):30-32
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changing of T4 5'-and 5-deiodinase within rat brain under various iodin-nutritional states.
METHODSAnimal model of iodine-deficiency rat was performed and the rats were divided into 4 groups by the intake of iodine-nutrition, and then killed at an age of 20 days. The thyroid hormones level in serum was measured by ELISA and the activity of T(4) 5'-and 5-deiodinase within brain was analyzed.
RESULTSIn less-iodine (LI) group,TT4 and FT4 were accounting for 3.5% of the neutral-iodine (NI) group's, and FT3 was 174.0% of NI group's (P < 0.05). In NI group,TT4 and FT4 were 114.5% and 127.7% of NI group's (P < 0.05). In high-iodine (HI) group, TT4 and FT4 were 61.86% and 62.0% of NI group's, and FT3 was 184.9% of NI group's (P < 0.05). In LI group, the activity of T4 5'-deiodinase tissue of per gram (1.95 +/- 0.32) ngT3.microgT4(-1).h was significantly higher than that of NI group (P < 0.05), and the activity of 5-deiodinase (1.38 +/- 0.21) ngrT3.microg T4(-1).h(-1) is significantly less than that of NI group (1.59 +/- 0.23) (P < 0.05). In HI group the activity of T4 5'-and 5-deiodinase tissue of per gram (1.12 +/- 0.19 and 1.73 +/- 0.36) ngrT3.microgT4(-1).h(-1)was significantly less than that of NI group (P < 0.05).
CONCLUSIONThe activity of T4 5'-deiodinase in iodine deficiency heightens and that in iodine excess is debased, the activity of T4 5-deiodinase in iodine deficiency and in iodine excess is debased.
