Effect of conjugated linoleic acid on gene expression of adiponectin of obese rat fed with high fat diet.
- Author:
Xiao-rong ZHOU
1
;
Chang-hao SUN
;
Hai-ying WANG
;
Li-ying JIANG
;
Rong LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adiponectin; biosynthesis; genetics; Adipose Tissue; metabolism; Animals; Insulin Resistance; physiology; Linoleic Acids, Conjugated; pharmacology; Male; Obesity; metabolism; PPAR gamma; biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Preventive Medicine 2005;39(1):33-36
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of conjugated linoleic acid (CLA) on expression of adiponectin in white adipose tissue of obese rats.
METHODSMale Wistar rats were randomly divided into control group, high-fat group and high fat + CLA group (0.75 g, 1.50 g, 3.00 g per hundred gram diet weight), we observed the effect of CLA on serum insulin and glucose levels of obese rats, and the reverse transcription polymerase chain reaction (RT-PCR) technique was used to measure the expression level of adiponectin and peroxisome proliferator-activated receptor-gamma (PPARgamma) mRNA.
RESULTSThe serum insulin and glucose levels of obese rats were (11.11 +/- 2.73) microIU/ml, (5.09 +/- 0.66) mmol/L. The supplement of CLA decreased the hyperinsulinemia and hyperglycemia, the serum insulin in CLA group (0.75 g, 1.50 g, 3.00 g per hundred gram diet weight) were (6.99 +/- 1.77) microIU/ml, (7.36 +/- 1.48) microIU/ml, (7.85 +/- 1.60) microIU/ml (P < 0.05), and glucose were (4.28 +/- 0.72) mmol/L, (4.18 +/- 0.55) mmol/L (P < 0.05), (4.06 +/- 0.63) mmol/L (P < 0.05), CLA can increase the expression of adiponectin and PPARgamma in adipose tissue of obese rat.
CONCLUSIONThe CLA might improve the insulin resistance of the obese rat and increase the expression of adiponectin mRNA, which might possibly act through activating PPARgamma.
