Two-dimensional zebrafish model combined with hyphenated chromatographic techniques for evaluation anti-osteoporosis activity of epimendin A and its metabolite baohuoside I.
- Author:
Yang ZHAN
;
Ying-Jie WEI
;
E SUN
;
Feng-Juan XU
;
Xiao-Bin JIA
- Publication Type:Journal Article
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Drug Evaluation, Preclinical;
Drugs, Chinese Herbal;
pharmacology;
Flavonoids;
metabolism;
pharmacology;
Mass Spectrometry;
Osteoporosis;
drug therapy;
Zebrafish
- From:
Acta Pharmaceutica Sinica
2014;49(6):932-937
- CountryChina
- Language:Chinese
-
Abstract:
This article firstly established a new efficient method for screening anti-osteoporosis ingredients, which used two-dimensional zebrafish model combined with hyphenated chromatographic techniques to evaluate anti-osteoporosis activities of epimedin A and its metabolite baohuoside I. Adult zebrafish was used for metabolism of epimedin A in 0.5% DMSO, and LC-MS was used for analysis of the metabolite, which was captured by HPLC, and prednisolone-induced osteoporosis model of zebrafish was used to evaluate the anti-osteoporotic activities of trace amounts of epimedin A and baohuoside I. The results indicated that epimedin A and baohuoside I can prevent prednisolone-induced osteoporosis in zebrafish. The developed method in this paper enables the separation, enrichment and analysis of micro-amount metabolite of epimedin A, and anti-osteoporosis activities in vivo of epimedin A and baohuoside I was simple and efficient screening resorting to zebrafish osteoporosis mode. This paper would provide new ideas and methods for a rapid and early discovery of anti-osteoporosis activities of micro-ingredients and its metabolite of traditional Chinese medicine.
