Effect of estradiol on cholesterol metabolism in J774a.1 mouse mononuclear/macrophage cells.
- Author:
Xue WANG
;
Jun LIU
;
Wen-Li DUAN
;
Jing SHANG
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Line;
Cholesterol;
metabolism;
Cholesterol Esters;
metabolism;
Estradiol;
pharmacology;
Foam Cells;
cytology;
metabolism;
Lipoproteins, LDL;
metabolism;
Macrophages;
drug effects;
metabolism;
Mice;
Scavenger Receptors, Class B;
metabolism
- From:
Acta Pharmaceutica Sinica
2014;49(7):1013-1018
- CountryChina
- Language:Chinese
-
Abstract:
To explore the anti-atherosclerotic mechanism of estrogen and especially observe the effect of estradiol on the content of cholesterol in J774a.1 mouse mononuclear/macrophage-derived foam cells which were incubated with oxidized low-density lipoproteins (ox-LDL). J774a.1 mouse mononuclear/macrophages were incubated with ox-LDL or with both ox-LDL and estradiol (1, 0.1 or 0.01 micromol x L(-1)). Oil red O staining was used to observe the formation of foam cells, and cholesterol oxidase fluorometric was used to determine the content of cellular cholesterol content. Western blotting and RTFQ-PCR were used to observe the expressions of scavenger receptor class B type I (SR-B I ) in J774a.1 foam cells. Compared with the control cells, J774a.1 mouse mononuclear/macrophage-derived foam cells showed significantly increased contents of total cholesterol and cholesterol ester (P < 0.001) and decreased SR-B I mRNA expression (P < 0.01). Estradiol treatment significantly lowered the contents of total cholesterol and cholesterol ester (P < 0.05), and increased SR-B I protein and mRNA expression (P < 0.01) in the foam cells in a dose-dependent manner. Estradiol can inhibit the formation of mononuclear/macrophage-derived foam cells by decreasing the contents of total cholesterol and cholesterol ester and up-regulating the expression of SR-B I in the foam cells.
