Comparison of structural characteristics and anticoagulation activity of enoxaparin sodium with different degree of 1,6-anhydro derivatives.
- Author:
He ZHU
;
You-Jing LIÜ
;
Xian-Wei HAN
;
Shi-Long LIU
;
Jie-Jie HAO
;
Xiao-Liang ZHAO
;
Guang-Li YU
- Publication Type:Journal Article
- MeSH:
Anticoagulants;
chemistry;
Enoxaparin;
chemistry;
Factor Xa Inhibitors;
chemistry;
Thrombin;
antagonists & inhibitors
- From:
Acta Pharmaceutica Sinica
2014;49(7):1049-1053
- CountryChina
- Language:Chinese
-
Abstract:
The fine structure of enoxaparin sodium samples with different degree of 1,6-anhydro derivatives were analyzed with polyacrylamide gel electrophoresis, high performance liquid chromatography, ultraviolet spectroscopy, infrared spectroscopy and nuclear magnetic resonance spectroscopy. A further study of anticoagulation activity of enoxaparins was performed, including those on their inhibition activities of coagulation factor Xa (FXa) and thrombin (FIIa). The results showed that the anti-FXa and -FIIa activities of enoxaparins with different degree of 1,6-anhydro derivatives (20.0%-39.7%) with similar structure characteristics, had decreasing tendency when the degree of 1,6-anhydro derivatives increased. Especially, the anti-FXa activity was sensitive to the change of the degree of 1,6-anhydro derivatives.
