Influence of cationic cyclopeptide on microstructure and permeability of Caco-2 cell membrane.
- Author:
Xiao-Hui LI
;
Ming-Ming CHANG
;
Qing WANG
;
Yu-Ming SUN
;
Yang LIU
- Publication Type:Journal Article
- MeSH:
Caco-2 Cells;
Cations;
Cell Membrane;
drug effects;
Cell Membrane Permeability;
drug effects;
Humans;
Insulin;
metabolism;
Membrane Fluidity;
drug effects;
Microscopy, Atomic Force;
Peptides, Cyclic;
pharmacology
- From:
Acta Pharmaceutica Sinica
2014;49(7):1062-1068
- CountryChina
- Language:Chinese
-
Abstract:
The microstructure of cationic cyclopeptide (TD-34) treated Caco-2 cell membrane was observed, and we discussed the relationship between membrane structure and insulin transmembrane permeability. Atomic force microscope (AFM) was used to observe living cell membrane in air condition and tapping mode. Results showed that the surface of Caco-2 cell membrane treated with TD-34 lost its smoothness and nearly doubled its roughness. Apparent permeability coefficients (P(app)) of insulin in Caco-2 cell monolayers increased 2.5 times. In conclusion, AFM can be used to observe microstructure of cationic cyclopeptide treated cell membrane and cationic cyclopeptide enhanced insulin delivery across Caco-2 cell membrane by increasing membrane fluidity.
