Neuroprotective effects of the effective components group of xiaoshuantongluo against oxygen-glucose deprivation in primary cultured rat cortical neurons.
- Author:
Xin-Mei XIE
;
Xiao-Bin PANG
;
Yan ZHAO
;
Bao-Quan WANG
;
Ruo-Yun CHEN
;
Guan-Hua DU
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Cell Survival;
Cells, Cultured;
Drugs, Chinese Herbal;
pharmacology;
Glucose;
Janus Kinase 2;
metabolism;
Neurons;
drug effects;
metabolism;
Neuroprotective Agents;
pharmacology;
Oxygen;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Rats;
STAT3 Transcription Factor;
metabolism;
Signal Transduction;
bcl-2-Associated X Protein;
metabolism
- From:
Acta Pharmaceutica Sinica
2014;49(8):1130-1135
- CountryChina
- Language:Chinese
-
Abstract:
This study is to investigate the effect of the effective components group of Xiaoshuantongluo (XECG) on neuronal injury induced by oxygen-glucose deprivation (OGD) in primary cortical cultures isolated from SD rat cortex at day 3 and the possible mechanism. Cells were divided into control group, OGD model group and XECG group (1, 3 and 10 mg x L(-1)). The cell viability was assessed with MTT assay and the LDH release rate was measured by enzyme label kit. The cell apoptosis was analyzed using Hoechst staining. RT-PCR was applied to detect the mRNA levels of JAK2 and STAT3. Western blotting was used to detect the expressions of Bcl-2, Bax, p-JAK2 and p-STAT3 proteins. Results showed that XECG resulted in an obvious resistance to oxygen-glucose deprivation-induced cell apoptosis and decrement of cell viability, decrease the cell LDH release rate. XECG could adjust the expression of Bcl-2 and Bax proteins and increase Bcl-2/Bax ratio, up-regulate the expression of p-JAK2 and p-STAT3. In conclusion, XECG could protect against the neuronal injury cells exposed to OGD, which may be relevant to the promotion of JAK2/STAT3 signaling pathway, and impact the expression of Bax and Bcl-2.
