Effects of Enalapril on Synthesis of Collagen TypeI, II, III in Incomplete Ureteral Obstruction with Uninephrectomized Rat.
- Author:
Joong Ho KIM
;
Yong Seok LEE
;
Myung Sik SIN
;
Jong Yook LEE
;
Seong Il SEO
;
Joon Chul KIM
;
Tae Kon HWANG
- Publication Type:Original Article
- Keywords:
collagen;
Nephrectomy;
Ureteral obstruthion;
ACE inhibitor
- MeSH:
Angiotensin II;
Angiotensins;
Animals;
Collagen Type I;
Collagen*;
Enalapril*;
Fibrosis;
Kidney;
Nephrectomy;
Rats*;
United Nations;
Ureter*;
Ureteral Obstruction*
- From:Korean Journal of Urology
2001;42(1):1-9
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Unilateral ureteral obstruction results in tubulointerstitial fibrosis of the affected kidney which is driven by increased levels of angiotensin II. In this study, we examed the fibrotic changes in un inephrectomized rats with incomplete urteral obstruction and explored the effect of an angiotensin converting enzyme(ACE)inhibitor, enalapril on the tubulointerstitial fibrosis of obstructive uropathy. MATERIALS AND METHODS: 130 Rats were sacrificed at 1, 2, 4 weeks after nephrectomy or obstruction. Collagen type I, II, and III were localized by immunohistochemical staining. And the distribution of each collagen type was analyzed using differences of their staining densities. RESULTS: There is no difference in total collagen amount and immunohistochemical staining between control and uninephrectomized group. After 2weeks in incomplete ureteral obstruction in uninephretomized rat, the total amount of collagen in affected kidney was significantly increased compared to control (p<0.05), and immunohistochemical staining for all type of collagen was gradually increased by increased duration of incomplete ureteral obstruction. Enalapril did not affect the total collagen amount and immunohistochemical staining in the kidney of uninephrectomized rat. Enalapril significantly decreased the collagen amount in affected kidneys of collagen was not significantly changed compaired to control group. CONCLUSIONS: the incomplete ureteral obstruction in uninephrectomized rat induces progressive increase of amount of collagen according to the duration of obstruction, especially 2 weeks, and enalapril administration after incomplete unilateral obstruction of tubulointerstitial fibrosis of obstructive uropathy.
