1H NMR based metabolomics study of bu-zhong-yi-qi-tang in the spleen-qi deficiency rat model.
- Author:
Lei CHEN
;
Huan XIANG
;
Jie XING
;
Jun-Sheng TIAN
;
Xue-Mei QIN
;
Guan-Hua DU
- Publication Type:Journal Article
- MeSH:
Administration, Oral;
Animals;
Disease Models, Animal;
Drugs, Chinese Herbal;
pharmacology;
Medicine, Chinese Traditional;
Metabolomics;
Proton Magnetic Resonance Spectroscopy;
Qi;
Rats;
Spleen
- From:
Acta Pharmaceutica Sinica
2014;49(9):1320-1325
- CountryChina
- Language:Chinese
-
Abstract:
The present study aimed to investigate the effect and the mechanisms of Bu-zhong-yi-qi-tang (BZYQ) on Spleen-Qi deficiency rat's model using nuclear magnetic resonance (NMR) metabolomics and multivariate statistical analysis methods. The rat Spleen-Qi deficiency model was established as follows: oral administration of Radix Rhei extract, loaded swimming and starvation for 24 h. The body weight and motor behavior of the rats were measured and recorded once a week. BZYQ could significantly improve body weight and behavioral of Spleen-Qi deficiency model rats compared with the model group (P < 0.05, 0.01). After drug administration, the changes in the levels of endogenous metabolites in the spleen including decreasing lactate, taurine and hypoxanthine, increasing glutamate and scyllo-inositol compared with the model group. The metabolomics approach is an effective tool for the investigation of the pharmacologic mechanism of BZYQ and it is helpful to further research.
