Comparison of the chemical composition between fresh and dried venenum bufonis by UPLC-Orbitrap MS.
- Author:
Jing HE
1
;
Yan LI
;
Nan SI
;
Hai-Yu ZHAO
;
Bao-Lin BIAN
;
Hong-Jie WANG
Author Information
1. Institute of Chinese Materia Medica, Chinese Academy of Medical Sciences, Beijing, China.
- Publication Type:Journal Article
- MeSH:
Amphibian Venoms;
chemistry;
Animals;
Bufanolides;
chemistry;
Bufonidae;
Chromatography, High Pressure Liquid;
Tandem Mass Spectrometry
- From:
Acta Pharmaceutica Sinica
2014;49(10):1446-1450
- CountryChina
- Language:Chinese
-
Abstract:
To identify the active components in Bufo melanostictus Schneider and clarify the difference between fresh and dried Venenum Bufonis, a UPLC-Orbitrap MS method has been established. The separation was performed with gradient elution of acetonitrile and water (with 0.1% formic acid) as mobile phase. By comparing their retention time and high resolution mass data of Venenum Bufonis extracts, 39 effective components were primarily identified by MS/MS analysis in positive ion mode. Twenty-six of them were bufadienolides. There were significant differences in the main composition between fresh and dried Venenum Bufonis. There are fewer bufadienolides in fresh toad venom.
