Interaction of E3 ligase HUWE1 and eukaryotic translation initiation factor eIF4E.
- Author:
Jun-Ping ZHANG
;
Ai-Juan XIA
;
Rui-An XU
- Publication Type:Journal Article
- MeSH:
Animals;
Eukaryotic Initiation Factor-4E;
metabolism;
Humans;
Ubiquitin-Protein Ligases;
metabolism
- From:
Acta Pharmaceutica Sinica
2014;49(11):1543-1546
- CountryChina
- Language:Chinese
-
Abstract:
To explore the regulation of eIF4E, we screened the protein interacting with eIF4E from human cDNA library by using yeast two-hybrid system. Several clones interacting with eIF4E were identified. One of them was homologous with HUWE1 (HECT, UBA and WWE domain containing 1, also named as ARF-BP1, HECTH9 or HUWE1). Cell co-immunoprecipitation showed that eIF4E could bind to HUWE1 in mammalian cells. We also found that HUWE1 bearing the HECT domain is necessary for its association with eIF4E.
