Effect of the chelator Zn-DTPA on the excretion of lead in lead intoxication mice detected with ICP-MS.
- Author:
Chen LI
;
Kai-zhi LU
;
Qi ZHOU
;
Qiong WANG
;
Yu-liang ZENG
;
Hong-jun YIN
;
Xuan-hui HE
;
Ying TIAN
;
Jun-Xing DONG
- Publication Type:Journal Article
- MeSH:
Animals;
Chelating Agents;
pharmacology;
Lead;
pharmacokinetics;
urine;
Lead Poisoning;
drug therapy;
Mass Spectrometry;
Mice;
Pentetic Acid;
pharmacology
- From:
Acta Pharmaceutica Sinica
2014;49(11):1588-1592
- CountryChina
- Language:Chinese
-
Abstract:
To study the lead excretion effect of the chelator Zn-DTPA on the lead intoxication mice, inductively coupled plasma mass spectrometry (ICP-MS) was applied to detect the lead content of biological samples. The acute lead intoxication mice model was established by injecting lead acetate intraperitoneally with the dose of 1 mg. Zn-DTPA was administered intraperitoneally to mice once daily for five consecutive days 4 h after intoxication. Control group, model group, combination of Zn-DTPA and Ca-DTPA group were evaluated at the same time. The urine was collected every day. The mice were sacrificed in batches in the 2rd, 4th, 6th day. Biological samples including urine, whole blood, femur and brain were prepared and nitrated. Lead concentration was detected by ICP-MS. The result showed that Zn-DTPA could increase lead content in urine markedly and reduce lead content in blood, femur and brain.
