Determination of γ-aminobutyric acid in human plasma by LC-MS/MS and its preliminary application to a human pharmacokinetic study.
- Author:
Yao CHEN
;
Xiao-Jian DAI
;
Jiang-Bo DU
;
Kan ZHONG
;
Xiao-Yan CHEN
;
Da-Fang ZHONG
- Publication Type:Journal Article
- MeSH:
Chromatography, Liquid;
Humans;
Tandem Mass Spectrometry;
gamma-Aminobutyric Acid;
blood
- From:
Acta Pharmaceutica Sinica
2014;49(11):1593-1599
- CountryChina
- Language:Chinese
-
Abstract:
A rapid, sensitive and convenient LC-MS/MS method was developed for the determination of γ-aminobutyric acid (GABA) in human plasma. d2-γ-Aminobutyric acid (d2-GABA) was synthesized as internal standard (IS). After extraction from human plasma by protein precipitation with acetonitrile, all analytes were separated on a Luna HILIC column (100 mm x 3.0 mm, 3 μm) using an isocratic mobile phase of water: acetonitrile: formic acid (20 : 80 : 0.12) with a flow rate of 0.5 mL x min(-1). Acquisition of mass spectrometric data was performed in multiple reaction monitoring mode (MRM) in positive electrospray ionization using the transitions of m/z 104 --> 69 for GABA and m/z 106 --> 71 for d2-GABA. The method was linear in the concentration range of 5.00 to 1 000 ng x mL(-1). The intra- and inter-day precisions were within 9.9%, and accuracy ranged from 99.1% to 104%, within the acceptable limit across all concentrations. The method was successfully applied to a pharmacokinetic study of GABA tablets in healthy Chinese volunteers.
