Experimental study on apoptosis induced by aqueous extract of Taxus chinensis in human pulmonary carcinoma cell A549 and its molecular mechanisms.
- Author:
Qi-jin SHU
1
;
Ping LI
;
Bin-bin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Extracellular Signal-Regulated MAP Kinases; metabolism; Humans; Inhibitor of Apoptosis Proteins; metabolism; Lung Neoplasms; pathology; MAP Kinase Signaling System; Plant Extracts; pharmacology; Taxus
- From: Chinese Journal of Integrated Traditional and Western Medicine 2011;31(9):1243-1247
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the apoptosis inducing effects of aqueous extract of Taxus chinensis (AETC) in human lung cancer cell A549 and its molecular mechanisms.
METHODSUsing MTT assay, the inhibition of AETC on the proliferation of A549 cells was detected. The apoptosis was detected by light and electron microscopy, and Annexin V-FITC/PI staining. The expressions of ERK1/2, JNK1/2, and survivin were analyzed by Western blot.
RESULTSAETC could inhibit the proliferation of A549 cells. Obvious apoptotic cells could be seen under light and electron microscope in AETC treated A549 cells. AETC's induction on the cell apoptosis was further confirmed by Annexin V-FITC/PI labeled quantitative detection. Western blot assay showed that AETC could decrease the survivin expressions. AETC showed no effects on ERK1/2 or JNK1/2 protein expressions.
CONCLUSIONAETC could significantly inhibit the proliferation of A549 cells and induce the apoptosis, which was mainly achieved through regulating the expressions of survivin.
