Effect of salvianolic acid B on TGF-beta1-induced human embryonic lung fibroblast's biological behavior.
- Author:
Min ZHANG
1
;
Zhi-shun FENG
;
Zhe-tong DENG
Author Information
- Publication Type:Journal Article
- MeSH: Benzofurans; pharmacology; Cell Proliferation; drug effects; Cells, Cultured; Collagen Type I; metabolism; Fibroblasts; cytology; drug effects; Humans; Lung; cytology; embryology; Transforming Growth Factor beta1; pharmacology
- From: Chinese Journal of Integrated Traditional and Western Medicine 2011;31(10):1401-1404
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of salvianolic acid B (SAB) on the proliferation of human embryonic lung fibroblast MRC-5, and the secretion of procollagen I and endogenous transforming growth factor-beta1, (TGF-beta1).
METHODSThe MRC-5 cells were randomly divided into four groups as follows: the control group: cells cultured with DMEM but with no TGF-beta1, or SAB; the TGF-beta1, group: cell cultured with 10 ng/mL TGF-beta1; the SAB1 group: cell cultured with medium with 10 ng/mL TGF-beta1 and 1 pmol/L SAB; the SAB2 group: cell cultured with medium with 10 ng/mL TGF-beta1, and 10 pmol/L SAB. The proliferation of cells was assayed by MTT incorporation. The concentration of amino-terminal propeptide of type I procollagen (PINP), a marker of collagen synthesis, was measured by radioimmunoassay. The endogenous TGF-beta1, levels were measured using ELISA.
RESULTSThe optical density, procollagen I contents, and endogenous TGF-beta1, levels significantly increased when compared with those of the control group (P<0.05). Compared with the TGF-beta1, group, the optical density was obviously lowered, the procollagen I contents and endogenous TGF-beta1, levels significantly decreased in the SAB1 group and the SAB2 group, and better in the SAB2 group, showing statistical difference (P<0.05).
CONCLUSIONSSAB could inhibit the proliferation of MRC-5 cells induced by TGF-beta1 and attenuate the roles of secreting collagen and endogenous TGF-beta1. It had the potential of postponing or delaying the progressive developing of pulmonary fibrosis.