Effect of erythromycin on apoptosis of epithelial cell in nasal polyps.
- Author:
Sai-Nan XU
1
;
Yu FENG
;
Yu ZHANG
;
Yi-Fei ZHOU
;
Lei YANG
;
Yong-Guo JIN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Apoptosis; drug effects; Case-Control Studies; Cell Line; Epithelial Cells; drug effects; metabolism; Erythromycin; pharmacology; Female; Humans; Male; Middle Aged; Nasal Polyps; metabolism; pathology; Proto-Oncogene Proteins c-bcl-2; metabolism; Young Adult; bcl-2-Associated X Protein; metabolism
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2006;41(7):510-513
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of erythromycin on apoptosis and expression of Bax and Bcl-2 of epithelial cell in nasal polyps.
METHODSEpithelial cells from thirty nasal polyps and fifteen inferior turbinates were cultured in Dulbecco Eagle and Ham F12 (1:1) and divided into two groups (one group were treated with Erythromycin). Apoptosis was detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling and expression of Bcl-2 and Bax detected by immunohistochemistry at 1, 3, 5 days after culture.
RESULTSThe apoptosis indexes (AI) of epithelial cell in nasal polyps after cultured for 1, 3, 5 days with erythromycin were (33.23 +/- 6.50)%, (38.21 +/- 7.22)% and (52.63 +/- 7.86)% respectively. The AI of epithelial cell in inferior turbinates were (31.02 +/- 5.60)%, (32.13 +/- 7.15)% and (39.64 +/- 7.48)% respectively. There was significant difference between two groups at 5 day after culture (P < 0.05). Expression of Bax and Bcl-2 of epithelial cell was significantly higher in nasal polyps than that in inferior turbinates (P < 0.01). Expression of Bax of epithelial cell cultured with erythromycin was significantly higher than that in the controls (P < 0.05). Apoptosis was clearly promoted after 5 day culture with Erythromycin (P < 0.05).
CONCLUSIONSErythromycin promoted expression of Bax and apoptosis of epithelial cell in nasal polyps.
