Inhibitory effects of small interfering RNA specific to protein kinase CK2a on the growth of laryngeal carcinoma cells.
- Author:
Jian-ting WANG
1
;
Shu-sheng GONG
;
Ying-peng LIU
;
Guang-li CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma; genetics; pathology; Casein Kinase II; genetics; Cell Line, Tumor; Cell Proliferation; Humans; Laryngeal Neoplasms; genetics; pathology; Plasmids; RNA, Messenger; genetics; RNA, Small Interfering; Transfection
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2006;41(8):617-621
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the effect of small interfering RNA (siRNA) specific to protein kinase CK2a on proliferation and apoptosis of Hep-2 cell line.
METHODSsiRNA expression plasmid psiRNA-hH1neo-CK2 specific to protein kinase CK2a and non-specific siRNA expression plasmid psiRNA-hH1neo-cont were constructed respectively, and then were transfected into Hep-2 cells by lipofectamine methods. Protein kinase CK2a mRNA and protein of the transfected cells were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western Blot, respectively. Proliferation and apoptosis of the transfected cells were observed by methyl thiazolyl tetrazolium (MTT) method and flow cytometry (FCM), respectively.
RESULTSProtein kinase CK2a mRNA and protein expressions were significantly decreased in the cells transfected with psiRNA-hH1neo-CK2 (P < 0.05). The Hep-2 cells grew slowly after transfected with psiRNA-hH1neo-CK2(P < 0.05). Obvious subdiploid peaks were found in the cells transfected with psiRNA-hH1neo-CK2 (P < 0.05).
CONCLUSIONSsiRNA expression plasmid specific to protein kinase CK2a suppressed the protein kinase CK2a expression and the proliferation of Hep-2, and induced apoptosis of Hep-2 cells.
