Possible Therapeutic Effect of Stem Cell in Atherosclerosis in Albino Rats. A Histological and Immunohistochemical Study.
10.15283/ijsc.2015.8.2.200
- Author:
Samraa H ABDEL-KAWI
1
;
Khalid S HASHEM
Author Information
1. Department of Histology, Faculty of Medicine, Beni-Suef University, Beni-suef, Egypt. samraahussein@yahoo.com
- Publication Type:Original Article
- Keywords:
Mesenchymal stem cells;
Atherosclerosis;
Cord blood;
eNOS;
iNOS
- MeSH:
Adult;
Animals;
Atherosclerosis*;
Cause of Death;
Cholesterol;
Collagen;
Diet;
Fetal Blood;
Humans;
Male;
Mesenchymal Stromal Cells;
Muscle, Smooth;
Rats*;
Stem Cells*;
Tail;
Vascular Diseases;
Veins;
Vimentin;
Vitamin D
- From:International Journal of Stem Cells
2015;8(2):200-208
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Atherosclerosis is the leading cause of death worldwide. there are no effective approaches to regressing atherosclerosis due to not fully understood mechanisms. Recently, stem cell-based therapies have held promises to various diseases, including vascular diseases. AIM: The present study aimed at investigating the possible effect of cord blood mesenchymal stem cell (MSC) therapy on atherosclerosis. MATERIAL AND METHODS: Eighty adult male albino rats were divided into control group (I), atherogenic group (II): subjected to high cholesterol fed diet (200~300 mg/kg body weight) for 12 weeks and 1.8 million units of vitamin D / kg of diet for 6 weeks. Stem cell therapy group (III): injected with stem cells in the tail vein following confirmation of atherosclerosis. Histological, Immunohistochemical and morphometric studies were performed were conducted. RESULTS: Atherogenic group (II) showed increased aortic thickness, intimal proliferation, smooth muscle proliferation and migration. Increased area % of collagen fibers, iNOS and vimentin immunoreactions were recorded and proved morphometrically. All findings regressed on stem cell therapy. CONCLUSION: A definite therapeutic effect of mesenchymal stem cells was found on atherosclerosis.
