Identification and expression of non-coding RNAs NC28 and NC119 in human tumors.
- Author:
Zhen-yuan SUN
1
;
Yan-hua YUAN
;
Chang-ning LIU
;
Yi ZHAO
;
Jie-fu HUANG
;
Xin-ting SANG
;
Yi-lei MAO
;
Xin LU
;
Yu WANG
;
Yong-sheng CHANG
;
Hai-tao ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Humans; Neoplasms; metabolism; RNA, Untranslated; biosynthesis
- From: Acta Academiae Medicinae Sinicae 2007;29(5):618-621
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore and identify the non-coding RNAs related to tumors.
METHODSWe used RT-PCR and Northern blot to analyze non-coding RNAs in tumor tissues and cell lines.
RESULTSTwo predicted non-coding RNAs were confirmed to be expressed in cancer tissues and cell lines by RT-PCR and DNA sequencing. We detected the expression of two non-coding RNA transcripts by Northern blot. The length of NC28 was about 1800 nt, and that of NC119 was about 1200nt.
CONCLUSIONSNC28 and NC119 have a tumor-associated expression pattern. The non-coding RNAs may play a role in the development of tumors.
