Effects of Maternal Serum on Permeability of Glomerular Endothelial Cell Membrane
10.1007/sl1596-011-0143-7
- Author:
DU HUI
1
;
LIU HAIYI
;
ZHAO JUN
;
WU YUANYUAN
;
GONG XUN
;
ZHOU QIONG
;
Xu JINGJING
;
LI YUQI
;
SHI XINWEI
;
QIAO FUYUAN
Author Information
1. Department of Gynaecology and Obstetrics, Tongji Hospital, Tongji MedicalCollege, Huazhong University of Science and Technology, Wuhan 430030, China
- Keywords:
preeclampsia;
serum;
glomerular endothelial cells;
permeability;
sFlt-l;
VEGF
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2011;31(1):17-20
- CountryChina
- Language:Chinese
-
Abstract:
The mechanism of injury on the human glomerular endothelial cells (ciGENC) induced by preeclampsia serum was investigated.Concentration of maternal serum sFlt-1 protein was detected by ELISA.Fluorescently-labeled bovine serum albumin infiltrating through lower chamber of Transwell was measured by multifunction microplate reader.Morphologic change of ciGENC was observed under inverted phase contrast microscope.The concentration of sflt-1 in preeclampsia groups was significantly increased as compared with control group (P＜0.01).Permeability in preeclampsia groups was significantly increased as compared with control group (P＜0.01).By contrast with severe preeclampsia group,the permeability of ciGENC monolayer in mild preeclampsia group was decreased significantly (P＜0.05).Intervention of exogenous VEGF significantly decreased permeability of ciGENC in preeclampsia groups.It was concluded that sFlt-1 increased ciGENC permeability by damaging integrity of endothelial barrier function.
