Intervention of Nicotine on MNU-Induced Bladder Cancer in Rats
10.1007/sl1596-011-0159-z
- Author:
LIU DI
1
;
PAN FENG
;
LI BING
;
HAN XIAOMIN
;
LI WENCHENG
;
SHI YING
;
PANG ZILI
;
ZHANG QIJUN
Author Information
1. Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,China
- Keywords:
bladder cancer;
nicotine;
p53 protein
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2011;31(1):103-106
- CountryChina
- Language:Chinese
-
Abstract:
This study examined the effect of nicotine on the expression of mutant p53 (mt-p53) in bladder cancer rats.The rat models of bladder cancer were established by infusing N-methyl-nitroso-urea (MNU,10 mg/kg every 2 weeks for 8 weeks) into the bladder.Pathological examination on the bladder was conducted to confirm the establishment of the model.All the bladder cancer rats were randomly divided into an MNU group and 3 nicotine groups.In the nicotine groups,the rats were intragastrically administered nicotine at different concentrations (25,15,5 mg/kg respectively)3 times per week for 8 weeks.The mt-p53 expression was detected by the immunohistochemical method.The results showed that rat bladder cancer models developed histopathological changes of bladder transitional cell carcinoma.The positive rate of mt-p53 expression in the 3 nicotine groups (25,15,5 mg/kg) was 75.00％,58.33％ and 41.67％ by the 14th week,respectively,significantly higher than that in the MNU group (33.33％) (all P＜0.05).The mt-p53 expression rate was positively correlated with the medication dose and time (P＜0.05).It is concluded that nicotine may play an important role in the development of bladder cancer partially by increasing the expression of mt-p53.
