Contribution of Decreased Expression of Ku70 to Enhanced Radiosensitivity by Sodium Butyrate in Glioblastoma Cell Line (U251)
10.1007/sl1596-011-0381-8
- Author:
LI YUHUI
1
,
2
;
ZHOU HONGXIA
;
XING ENMING
;
Dassarath MEERA
;
REN JINGHUA
;
DONG XIAORONG
;
LIU HONGLI
;
YANG KUNYU
;
WU GANG
Author Information
1. Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430023, China
2. Department of Oncology, Wuhan Central Hospital, Wuhan 430014, China
- Keywords:
sodium butyrate;
radiosensitivity;
Ku70;
DNA double-strand breaks;
γ-H2AX
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2011;31(3):359-364
- CountryChina
- Language:Chinese
-
Abstract:
The present study investigated the enhanced radiosensitivity of U-251 cells induced by sodium butyrate (NaB) and its possible mechanisms.Increased radiosensitivity of U251 cells was examined by clonogenic cell survival assays.The expression of Ku70 mRNA and protein was detected by using RT-PCR and Western blotting respectively.γ-H2AX foci were measured at different time points after ionizing irradiation alone or combined with NaB treatment.The results showed that cell survival rate was significantly reduced,both D0 and Dq values were decreased (D0:1.43 Gy vs.1.76 Gy; Dq:1.22 Gy vs.2.05 Gy) after the combined treatment as compared with irradiation alone,and sensitivity enhancing ratio (SER) reached 1.23.The average number ofγ-H2AX foci per cell receiving the combined treatment was significantly increased at different time points,and the expression levels of Ku70mRNA and protein were suppressed by NaB in a dose-dependent manner.It was concluded that enhanced radiosensitivity induced by NaB involves an inhibited expression of Ku70 and an increase in γ-H2AX foci,which suggests decreased ability in DSB repair.
