Triptolide-induced Apoptosis by Inactivating Nuclear Factor-kappa B Apoptotic Pathway in Multiple Myeloma in vitro
10.1007/sl1596-011-0471-7
- Author:
ZENG RONG
1
;
ZENG LINLAN
;
CHEN YAH
;
ZHAO FEI
;
LI RUI
;
WEN LU
;
ZHANG CHUN
Author Information
1. Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022,China
- Keywords:
triptolide;
human multiple myeloma;
apoptosis;
NF-κB/p65;
IκBα
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2011;31(4):446-451
- CountryChina
- Language:Chinese
-
Abstract:
The effect of triptolide on proliferation and apoptosis of human multiple myeloma RPMI-8226 cells in vitro,as well as the roles of nuclear factor-kappa B (NF-κB) and IκBα was investigated.The effect of tritptolide on the growth of RPMI-8226 cells was studied by MTT assay.Apoptosis was detected by Hoechest 33258 staining and Annexin V/PI double staining assay.The expression of NF-κB and IκBα was observed by Western blot and confocal microscopy.The results showed that triptolide inactivated NF-κB apoptotic pathway in human multiple myeloma RPMI-8226 cells.Triptolide at nM range induced proliferation inhibition in a dose- and time-dependent manner and apoptosis in a dose-dependent fashion in RPMI-8226 cells.Besides,we observed the inhibition of NF-κB/p65 in the nuclear fraction was correlated with the increase in the protein expression of IκBα in the cytosol.These results suggested that triptolide might exhibit its strong anti-tumor effects via inactivation of NF-κB/p65 and IκBα.
