Expression of tumor suppressor gene PRDM1 in lung cancers.
- Author:
Yan-Yan LIU
1
;
Shu-Jun YANG
;
Jie MA
;
Li WANG
;
Yuan-Hua LIU
;
Sai-Juan CHEN
;
Zhi-Xiang SHEN
;
Wei-Li ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; genetics; metabolism; Adult; Aged; Biomarkers, Tumor; genetics; metabolism; Blotting, Western; Carcinoma, Small Cell; genetics; metabolism; Carcinoma, Squamous Cell; genetics; metabolism; Female; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Lung Neoplasms; genetics; metabolism; Male; Middle Aged; Positive Regulatory Domain I-Binding Factor 1; Repressor Proteins; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transcription Factors; genetics; metabolism
- From: Chinese Journal of Oncology 2007;29(6):434-436
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the expression of tumor suppressor gene PRDM1 in lung cancers.
METHODSForty-five cases were enrolled in this study, including squamous cell carcinoma (20 cases), adenocarcinoma (15 cases), and small cell cancer (10 cases). PRDM1 protein was detected in paraffin-embedded tissue by immunohistochemistry. Tumor cells in lung cancers were further selected by laser microdissection for RT-PCR analysis. PRDM1 protein in frozen tissue was also detected by Western blot.
RESULTS(1) PRDM1 protein was found in paraffin-embedded tissues in 90.0% (18/20) of squamous cell carcinoma, 13.3% (2/15) of adenocarcinoma, and 0 (0/10) small cell lung cancer. Squamous cell carcinoma predominantly expressed PRDM1 protein ( P < 0.01). (2) Gene product of PRDM1 DNA binding region was not found in microdissected tumor cells, but an abnormal PRDM1 protein about 70 KD was detected simultaneously in whole tumor tissue.
CONCLUSIONPRDM1 may be considered as a specific biomarker in pulmonary squamous cell carcinoma. The abnormal PRDM1 expression both at transcriptional and protein levels indicated that this tumor suppressor gene lost its function, which may become a new target in the strategy of treatment for lung cancers.
