Expression and relationship of p27(kip1) and its related molecules Jab1 and CRM1 during proliferation of lymphoma cells U937.
- Author:
Yu-Chan WANG
1
;
Dong-Mei ZHANG
;
Ai-Guo SHEN
;
Jian-Xing LU
;
Xiao-Yi SHAO
;
Song HE
;
Chun CHENG
Author Information
- Publication Type:Journal Article
- MeSH: COP9 Signalosome Complex; Cell Culture Techniques; Cell Nucleus; metabolism; Cell Proliferation; Culture Media, Serum-Free; pharmacology; Cyclin-Dependent Kinase Inhibitor p27; metabolism; Cytoplasm; metabolism; Humans; Intracellular Signaling Peptides and Proteins; metabolism; Karyopherins; metabolism; Peptide Hydrolases; metabolism; Receptors, Cytoplasmic and Nuclear; metabolism; U937 Cells
- From: Chinese Journal of Oncology 2007;29(9):657-661
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression and relationship of p27(kip1) and its related molecules Jab1 and CRM1 during proliferation of lymphoma cells U937.
METHODSU937 cells were treated with serum starvation and release, and the effects of these treatments on the cell growth was tested with cell number counting. The expression and localization of p27(kip1), Jab1 and CRM1 in U937 cells were detected by Western blot, double immunolabelling and laser scanning confocal microscopy.
RESULTSThe growth of U937 cells was blocked by serum starvation. The total protein of p27(kip1) was increased while Ser10-phosphorylated p27(kip1) -related molecules Jab1 and CRM1 were decreased. Meanwhile, the location of p27(kip1) was changed from cytoplasm into nuclei. After serum release, the location of p27(kip1) expression reappeared in the cytoplasm again.
CONCLUSIONDuring the proliferation process of lymphoma U937 cells, Jab1 and CRM1 may influence the location and expression of p27kip1, and may participate in regulation of growth of NHL cells.
