Expression and biological significance of HPA and HIF-1alpha in human esophageal squamous cell carcinoma.
- Author:
Jia-Lin LIU
1
;
Bing-Juan ZHOU
;
Ying ZHANG
;
Yan-Ping WU
;
Shu-Zhe LIU
;
Juan XUE
;
Wei-Hua LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Carcinoma, Squamous Cell; metabolism; pathology; Esophageal Neoplasms; metabolism; pathology; Esophagus; metabolism; pathology; Female; Gene Expression Regulation, Neoplastic; Glucuronidase; genetics; metabolism; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; genetics; metabolism; Immunohistochemistry; In Situ Hybridization; Lymphatic Metastasis; Male; Middle Aged; Mucous Membrane; metabolism; Neoplasm Invasiveness; Neoplasm Staging; RNA, Messenger; metabolism
- From: Chinese Journal of Oncology 2007;29(9):689-692
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of HPA and HIF-1alpha in human esophageal squamous cell carcinoma and their relationship with cancer development, invasion and metastasis.
METHODSThe expression of HPA mRNA and HIF-1alpha protein in 23 mucosa specimens of incisal margin, 26 para-tumorous dysplastic tissues and 70 esophageal squamous cell carcinoma specimens were detected by in situ hybridization assay and immunohistochemical staining, respectively.
RESULTSThe positive expression of HPA mRNA and HIF-1alpha protein were significantly increased as the epithelial cells progressed into carcinoma (P < 0.05). The expression of HPA mRNA and HIF-1alpha protein in the esophageal squamous cell carcinoma were significantly correlated with the invasion depth, lymph node metastasis and clinical staging (P < 0.05), while it was not correlated with the differentiation of tumors (P > 0.05). There was a close correlation between the expression of HPA mRNA and HIF-1alpha protein in the carcinoma tissues (P < 0.01).
CONCLUSIONThe high expression of HPA mRNA and HIF-1alpha protein is correlated with carcinogenesis, progression, invasion and metastasis of esophageal squamous cell carcinoma. There may be a positive cooperation between two of them in the carcinogenesis and development of esophageal carcinoma. The determination of HPA mRNA and HIF-1 alpha will be useful in predicting tumor biological behavior.
