Anti-tumor effects of chemotherapeutic drugs on human gastric cancer cells in vitro and the relationship with expression of hTERT mRNA.
- Author:
Ming GENG
1
;
Ying-Chun YIN
;
Yong-Cheng CAO
;
Zhi-Jie FU
;
Xing-You WANG
;
Yan-Hong TAI
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma, Mucinous; metabolism; pathology; Adenocarcinoma, Papillary; metabolism; pathology; Adult; Aged; Antibiotics, Antineoplastic; pharmacology; Antimetabolites, Antineoplastic; pharmacology; Antineoplastic Agents; pharmacology; Antineoplastic Agents, Phytogenic; pharmacology; Carcinoma, Signet Ring Cell; metabolism; pathology; Cell Proliferation; drug effects; Cisplatin; pharmacology; Doxorubicin; pharmacology; Drug Resistance, Neoplasm; Female; Fluorouracil; pharmacology; Humans; Male; Middle Aged; Mitomycin; pharmacology; Paclitaxel; pharmacology; RNA, Messenger; metabolism; Stomach Neoplasms; metabolism; pathology; Telomerase; genetics; metabolism
- From: Chinese Journal of Oncology 2007;29(11):838-841
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate in vitro antitumor effects of chemotherapeutic drugs, and investgate the relationship with expression of hTERT mRNA in human gastric cancer tissues.
METHODSFresh samples of gastric cancer obtained from operation room were prepared to single-cell suspension (3 x 10(5) to 5 x 10(5) cells ml(-1)) and were separately exposed to taxol (TAX), cisplatin (CDDP), 5-fluorouracil (5-Fu), adriamycin (ADM), mitomycin (MMC) for 48 hours. Metabolic activity and inhibitory rate of the cells were determined by trypan blue exclusion and MTT assay. Expression of hTERT mRNA was detected by in situ hybridization (ISH).
RESULTSThe inhibition rate of cancer cells exposed to chemotherapeutic drugs was different, and that of TAX, CDDP, 5-Fu was significantly higher than that of ADM and MMC. The positive rate of hTERT mRNA expression was 90.0% (54/60) and positive cells showed resistance to 5-Fu and ADM.
CONCLUSIONOverexpression of hTERT mRNA may contribute to primary drug-resistance of tumors. Chemosensitivity tests by MTT assay may contribute to prediction of effectness in applying chemotherapeutic drugs and identify drug-resistant cases.
