Study of molecular mechanism of tanshinone II A inducing differentiation in acute promyelocytic leukemia NB4 cells.
- Author:
Rui DU
1
;
Hong ZHENG
;
Yan-Ping WANG
;
Wen-Tong MENG
;
Hui QIN
;
Shu-Lan YUAN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Differentiation; drug effects; Cell Line, Tumor; Diterpenes, Abietane; Drugs, Chinese Herbal; pharmacology; Gene Expression Regulation, Leukemic; drug effects; Humans; Leukemia, Promyelocytic, Acute; drug therapy; genetics; metabolism; Phenanthrenes; pharmacology
- From: China Journal of Chinese Materia Medica 2008;33(24):2954-2958
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate molecular mechanism of tanshinone II A inducing differentiation and apoptosis in acute promyelocytic leukemia NB4 cells.
METHODNB4 cells were cultured in vitro and treated with tanshinone II A and observed cellular morphology, cell category and the cellular proliferation. DNA microarray technique was used to analyze the gene expression profiles of NB4 cells induced by tanshinone II A.
RESULT92.8% of NB4 cells treated with 0.5 mg x L(-1) tanshinone II A were induced into mature neutrophils, in which myetocytes and melamyetocytes were 27.0%, banded and segmented neutrophits 68.2%. Cell growth were inhibited. cDNA microarray showed the enormously expressed 183 genes including 23 differentiation associated genes, and other interrelated genes.
CONCLUSIONTanshinone II A inducing differentiation in NB4 cells may be via regulation of many kinds of genes, especially differentiation associated genes expression. This partially explained the molecular mechanism of tanshinone II A inducing differentiation.
