Study on preparation and quantity determination of Scrophularia ningpoensis dispensing granules.
- Author:
Zhengrui NIU
1
;
Rufeng WANG
;
Yu JING
;
Mingying SHANG
;
Shaoqing CAI
Author Information
- Publication Type:Journal Article
- MeSH: Calibration; Chromatography, High Pressure Liquid; Cinnamates; analysis; Drug Compounding; methods; standards; Drugs, Chinese Herbal; administration & dosage; chemistry; Glycosides; analysis; Linear Models; Pyrans; analysis; Quality Control; Reproducibility of Results; Scrophularia; chemistry; Sensitivity and Specificity; Solvents; chemistry; Temperature
- From: China Journal of Chinese Materia Medica 2009;34(1):39-42
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop the preparation method and to determine the quantity of effective constituents of Scrophularia ningpoensis dispensing granules.
METHODNine experiments were carried out through L9 (3(4)) orthogonal design. The contents of harpagoside and cinnamic acid were determined by HPLC method.
RESULTThe optimal extraction process was as follows: the slices were soaked in water in the ratio of 1:8 for 0.5 h, then they were decocted for 2 h. For the second time, 6 times of water was added and sustained for 1.5 h. Harpagoside and cinnamic acid were linear within the ranges of 0.0776-1.552 microg (r=0.9999) and 0.01446-0.4339 microg (r=0.9999), respectively. The average recoveries were 100.4% (RSD 1.98%, n=6) and 98.27% (RSD1.35%, n=6), respectively. Ten batches of granules were determined.
CONCLUSIONThe extraction process is scientific and the determination method is suitable for quality control of Scrophularia ningpoensis dispensing granules.
