Suppressive effects of GTW treatment on infiltration of inflammatory cell in glomeruli in anti-Thy1.1 glomerulonephritis.
- Author:
Yigang WAN
1
;
Wei SUN
;
Xiaoyan CHE
;
Haiming YANG
;
Ming GE
;
Wei DAI
;
Fujio SHIMIZU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; immunology; Dose-Response Relationship, Drug; Female; Gene Expression Regulation; drug effects; Glomerulonephritis; immunology; metabolism; pathology; physiopathology; Glycosides; pharmacology; Inflammation; metabolism; pathology; physiopathology; Interferon-alpha; genetics; Interleukin-2; genetics; Kidney Glomerulus; drug effects; pathology; Macrophages; drug effects; metabolism; RNA, Messenger; genetics; metabolism; Rats; T-Lymphocytes; drug effects; metabolism; Tripterygium; chemistry
- From: China Journal of Chinese Materia Medica 2009;34(1):72-77
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine inhibition action of multi-glycoside of Tripterygium wilfordii (GTW) on infiltration of inflammatory cell in glomeruli with anti-Thy1.1 glomerulonephritis (anti-Thy1.1 GN), and to clarify its effects on inflammatory in vitro.
METHODTwo types of anti-Thy1.1 GN were induced in rats by a single or two intravenous injections with 500 microg of anti-Thy1.1 mAb 1-22-3. Rats were randomly divided into two groups, the GTW group and control group, and sacrificed on day 7 or on day 42 after induction of anti-Thy1.1 GN. Daily oral administration of different dose of GTW and distilled water as a control was started from 3 days before injection or at the same time of injection till the day of sacrifice. Proteinuria was determined during days 7 or during days 42. Infiltration of macrophage and T lymphocyte in glomeruli and mRNA expression of interleukin (IL)-2 and interferon (IFN)-gamma in renal tissue were examined.
RESULTIncrease of infiltration of macrophage in reversible anti-Thy1.1 GN model, glomerular macrophage infiltration and IL-2 mRNA expansion were attenuated by higher dose of GTW (75 mg x kg(-1) x d(-1)), and increased accumulation of activated macrophage and T lymphocyte in irreversible anti-Thy1.1 GN model, accumulation of macrophage and T lymphocyte in glomeruli and mRNA expansion of IL-2 and IFN-gamma were decreased by middling dose of GTW (50 mg x kg(-1) x d(-1)) as well. Proteinuria was significantly ameliorated after GTW administration.
CONCLUSIONThe findings suggested that different dose of GTW can ameliorate infiltration of inflammatory cell in glomeruli with anti-Thy1.1 glomerulonephritis in vitro by decreasing the expression of IL-2 and IFN-gamma.
