Studies on application of ultra-filtration to purifying polysaccharides from Rhubarb.

Author: Yu SUN ¹ ; Yongchuan ZHOU ; Dexu CHEN
Author Information

1. Modern Engineering Center for Chinese Traditional Medicine, East China University of Science and Technology, Shanghai 200237, China.
Publication Type:Journal Article
MeSH: Feasibility Studies; Hydrogen-Ion Concentration; Polysaccharides; isolation & purification; Pressure; Rheum; chemistry; Temperature; Time Factors; Ultrafiltration; methods
From: China Journal of Chinese Materia Medica 2009;34(2):165-168
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo purify Rhubarb polysaccharides via micro-filtration and ultra-filtration.
METHODThe technology adopted micro-filtration and tubular membrane with different cut-off molecular weights.
RESULTExperiment results showed that the optimum operating conditions were that the ultra-filtration time controlled around 80 min, the temperature was set the 35-40 degrees C, the operating pressure was 0.08-0.12 MPa, the pH was 6-8, and a sample that of polysaccharide was 0.5 times of original concentration. The recovery ratio of the polysaccharides was 53.7%, the concentration could be condensed to 2.73 times compared with the sample liquid.
CONCLUSIONThe technology was simple and feasible, it can be applied to purify Rhubarb polysaccharides.