Study on C21 steroidal glycosides of Cynanchum atratum by LC-MS.
- Author:
Xinchao ZHAO
1
;
Hong BAI
;
Yongjun LIU
;
Wei LI
;
Yuanshu WANG
;
Aiqin LIU
;
Kazuo KOIKE
Author Information
1. Institute of Materia Medica, Shandong Academy of Medical Sciences, Key Laboratory for Mordern Medicine and Technology of Shandong Province, Jinan 250062, China.
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
Cynanchum;
chemistry;
Glycosides;
analysis;
chemistry;
Spectrometry, Mass, Electrospray Ionization;
Steroids;
chemistry;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2009;34(2):186-188
- CountryChina
- Language:Chinese
-
Abstract:
High performance liquid chromatography coupled with on-line electrospray tandem mass spectrometry (HPLC/ESI-MS/MS) was used to identify C21 steroidal glycosides in the roots of Cynanchum atratum. The structures of C21 steroidal glycosides were deduced from mass fragments features in positive and negative mode. The constituents of C. atratum were separated and detected. 7 compounds were identified by comparing their ESI-MS/MS data with the reference compounds and 2 compounds were inferred solely by the ESI-MS/MS data. The method is sensitive, and provides good separation and rapid qualitative characterization of C21 steroidal glycosides in the roots of C. atratum.
