Protective effects of AST and ASI on memory impairment and its mechanism in senescent rats treated by GC.
- Author:
Weizu LI
1
;
Weiping LI
;
Yanyan YIN
;
Huiling GONG
;
Guocui WU
;
Fenfang ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Aging; drug effects; metabolism; pathology; Animals; Apoptosis; drug effects; Body Weight; drug effects; Calcium; metabolism; Dexamethasone; adverse effects; Female; Glucocorticoids; adverse effects; Hippocampus; pathology; Intracellular Space; drug effects; metabolism; Male; Memory Disorders; chemically induced; metabolism; pathology; prevention & control; Neurons; drug effects; pathology; Rats; Saponins; pharmacology
- From: China Journal of Chinese Materia Medica 2009;34(2):199-203
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the protective effects and mechanisms of astragaloside (AST) and astragalus saponin I (ASI) on the memory impairment in senescent rats treated by glucocorticoid (GC).
METHODY maze test was performed to determine the effects of AST and ASI on memory impairment of hydrocortisone(HC)-induced senescent rats. Using Ca2+ sensitive fluorescent indicator (Furo-2), free intracellular calcium concentration ([Ca2+]i) was measured by double wavelength fluorescence sepectrophotometer in thymocytes and hippocampal neurons induced dexamethasone (DEX). And apoptosis was detected by DNA gel electrophoresis and flow cytometry.
RESULTCompared with HC control, AST and ASI can improve the memory of the senescent rats treated by HC, lower [Ca2+]i and suppress apoptosis of thymocytes and hippocampal neurons induced by DEX.
CONCLUSIONAST and ASI can delay the aging in rats treated by HC, and its mechanism may includ lowering[Ca2+]i and suppressing the apoptosis of thymocytes and hippocampal neurons.
