AFLP analysis of genetic diversity of Prunella.
- Author:
Yufeng SHEN
1
;
Yiming SUN
;
Xiaoxia SHEN
;
Jianming JIANG
;
Zhian WANG
;
Xuping YU
Author Information
- Publication Type:Journal Article
- MeSH: Amplified Fragment Length Polymorphism Analysis; DNA, Plant; analysis; Genetic Variation; Polymorphism, Genetic; Prunella; classification; genetics
- From: China Journal of Chinese Materia Medica 2009;34(3):260-263
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the variety of the genetic polymorphism of eight Prunella germplasm resources by AFLP analysis.
METHODThe amplified fragment length polymorphism (AFLP) tags were applied to screen out 32 selective amplification primer pairs, the amplified bands as original matrix were analyzed with NTSYS-PC software for the similarity between the Prunella germplasm and the construction of genetic phylogenetic tree.
RESULTSDS extraction of genomic Prunella DNA showed a good quality, could meet the requirements of AFLP analysis. From 32 selective amplification primer pairs, 10 pairs with strong polymorphism, better band and higher resolution were used for the construction of the AFLP Prunella fingerprint, all eight Prunella germplasms were separated, they were divided into 3 categories.
CONCLUSIONPrunella germplasm resources are rich in genetic diversity, certain morphological characteristics and differences are associate with genotype.
