RP-HPLC determination of tiliroside in Daphne genkwa.
- Author:
Lili SONG
1
;
Juan LU
;
Peifang YAN
;
Lina TAN
;
Xuwen LI
;
Yongri JIN
Author Information
- Publication Type:Journal Article
- MeSH: Acetonitriles; chemistry; Chromatography, High Pressure Liquid; methods; Daphne; chemistry; Flavonoids; isolation & purification; Oils, Volatile; pharmacology; Plant Stems; chemistry
- From: China Journal of Chinese Materia Medica 2009;34(3):301-303
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the contents of tiliroside [kaempferol 3-O-beta-D-(6 " -p-coumaroyl) glucopyranoside] in the Daphne genkwa.
METHODThe separation was performed on ZORBAX XDB C18 column (4.6 mm x 250 mm, 5 microm), using acetonitrile and water/acetic acid mixture (1,000/1) (gradient elution: 0-18 min, 26 : 74; 18-33 min, 80 : 20) as mobile phase with the rate of 1.0 mL x min(-1) at 25 degrees C, the wavelength for measurement was 310 nm.
RESULTThe linearity was in the range of 0.1-5.0 microg (n=6), and the correlation coefficient was 0.9999. The average recovery for compound tiliroside was 101.50% (RSD 0.90%) (n=6).
CONCLUSIONThis method is accurate, reliable and reproducible to determine tiliroside in the D. genkwa.
