Determination of oleuropein in stem of Syringa oblata from different districts by HPLC.
- Author:
Xiaohui WEI
1
;
Shuxia ZHANG
;
Weifeng ZHAI
;
Shujun ZHANG
;
Zhengtao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Acetonitriles; chemistry; Benzoates; chemistry; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; Iridoids; Pyrans; analysis; Syringa; chemistry; Vasodilator Agents; analysis
- From: China Journal of Chinese Materia Medica 2009;34(3):304-306
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for the determination of oleuropein in Syringa oblata.
METHODAn Aigilent ZORBAX SB- C18 column (4.6 mm x 250 mm, 5 microm) was used. The mobile phase was acetonitrile-water (21 : 79). The flow rate was 1.0 mL x min(-1). The detection wavelenghth was set at 232 nm and the column temperature was 30 degrees C.
RESULTThe linear range of oleuropein were from 0.011 62 g x L(-1) to 1.162 g x L(-1). The average recovery was 98.7% with RSD 2.5% (n=9).
CONCLUSIONThe method is reliable, accurate and specific. It can be used for quality control of the stem of Syringa oblata.
