Effects of 18beta-glycyrrhizic acid and 18alpha-glycyrrhizic acid on mRNA and protein expression of cytochrome P450 3A in cultured rat primary hepatocyte.
- Author:
Yuguang WANG
1
;
Minghui YANG
;
Zengchun MA
;
Qiande LIANG
;
Hongling TAN
;
Chengrong XIAO
;
Yue GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Clinical Laboratory Techniques; Cytochrome P-450 CYP3A; genetics; metabolism; Glycyrrhizic Acid; pharmacology; Hepatocytes; drug effects; metabolism; Protein Biosynthesis; drug effects; RNA, Messenger; metabolism; Rats; Rats, Sprague-Dawley; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; methods; Transcription, Genetic; drug effects; genetics
- From: China Journal of Chinese Materia Medica 2009;34(3):307-311
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of 18beta-glycyrrhizic acid and 18alpha-glycyrrhizic acid on mRNA and protein expression of cytochrome P450 3A in cultured rat primary hepatocyte.
METHODRat heaptocyte were isolated by two-step in situ collagenase perfusion method; the hepatocytes were seeded in dishes coated with type I rat tail collagen, culture medium was added and changed daily after gelation, the effect of 18beta-glycyrrhizic acid and 18alpha-glycyrrhizic acid on the expression of CYP3A was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot respectively.
RESULTTreatment with 18beta-glycyrrhizic acid of primary rat hepatocytes resulted in marked up-regulation of CYP3A1 expression at both mRNA and protein levels in a concentration dependent manner, while exposure to 18alpha-glycyrrhizic acid of primary rat hepatocytes resulted in marked down-regulation of CYP3A1 expression at both mRNA and protein levels in a concentration dependent manmer.
CONCLUSION18beta-glycyrrhizic acid and 18alpha-glycyrrhizic acid up-regulate and down-regulate CYP3A1 expression at the transcriptive levels.
