Study on preparation of berberine microemulsion and its absorption in intestine.
- Author:
Shuangying GUI
1
;
Lei WU
;
Jun PAN
;
Zhiqiang WEN
;
Weihua KAI
;
Jun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Absorption; Administration, Cutaneous; Animals; Berberine; administration & dosage; pharmacokinetics; Drug Delivery Systems; Drug Stability; Female; Ileum; metabolism; Intestinal Absorption; drug effects; Intestines; metabolism; Male; Particle Size; Rats; Rats, Wistar; Skin; metabolism; Skin Absorption; drug effects; physiology; Solubility; Technology, Pharmaceutical; methods
- From: China Journal of Chinese Materia Medica 2009;34(4):398-401
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare berberine microemulsion, and to investigate its properities and the absorption character in rat intestine in situ.
METHODThe optimum formulation of the blank microemulsion selected by pseudo tertiary phase diagrams and the berberine microemulsion was prepared based on the blank microemulsion. The viscosity, conductance, refraction rate and particle size of berberine microemulsion were surveyed. An in situ rat perfusion method was used to investigate the intestinal absorption of berberine microemulsion. A UV method for determination of berberine in the intestinal flux was established.
RESULTThe viscosity, conductance, refraction rate and particle size of berberine microemulsion were 2.11 cPas, 125.5 microomega, 1.363 and 24.0 nm, respectively. The absorption rate of berberine at the ileum was the best. The absorption of berberine microemulsion at the ileum was significantly higher than that of raw medicine (P < 0.01).
CONCLUSIONThe microemulsion system might improve the absorption of berberine in the intestinal tract.
