Alpha-glucosidase inhibitors from Luculia pinciana.
- Author:
Wenyi KANG
1
;
Li ZHANG
;
Yanli SONG
Author Information
- Publication Type:Journal Article
- MeSH: Acarbose; pharmacology; Coumarins; pharmacology; Enzyme Inhibitors; pharmacology; Glycoside Hydrolase Inhibitors; Inhibitory Concentration 50; Oleanolic Acid; isolation & purification; pharmacology; Plant Extracts; pharmacology; Rubiaceae; chemistry; Triterpenes; isolation & purification; pharmacology; alpha-Glucosidases; metabolism
- From: China Journal of Chinese Materia Medica 2009;34(4):406-409
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo search alpha-glucosidase inhibitors from Luculia pinciana.
METHODThe alpha-glucosidase inhibitor was isolated by the column chromatographic techniques and the bioassay-guided method in vitro. A combination of MS and NMR spectroscopy was used to identify the chemical structures. The inhibitory kinetic of the isolations was also investigated.
RESULTThe ethyl acetate extract showed strongest inhibitory activity, and five active compounds were isolated and identified as scopletin (1), 5-methoxy-8-hydroxycoumarin (2), lalpha,3beta,24-trihydroxyursolic acid (3), ursolic acid (4) and oleanolic acid (5). The IC50 values of all compounds were lower than that of acarbose. Four of them shown noncompetitive type manner on alpha-glucosidase inhibition except that compound 3 is competitive type manner.
CONCLUSIONCompounds 1-4 as the inhibitors of alpha-glucosidase were reported for the first time.
