The molecular epidemiologic investigation of hantavirus carried by rodent hosts in Wenzhou, Zhejiang province
10.3321/j.issn:0254-6450.2008.09.010
- VernacularTitle:浙江省温州市啮齿动物中汉坦病毒的分子流行病学研究
- Author:
Xian-Dan LIN
1
;
Peng-Fei YANG
;
Xiao-Wei LIAG
;
Mei-Fu LI
;
Na GAO
;
Yi CHEN
;
Shi-Dian ZENG
;
Huai-Jia WEN
;
Ling-Ping CHEN
;
Ming-Hui LI
;
Yong-Zhen ZHANG
Author Information
1. 浙江省温州市疾病预防控制中心
- Keywords:
Hantavirus;
Seoul virus;
Genotype;
Phylogenetic analysis
- From:
Chinese Journal of Epidemiology
2008;29(9):891-894
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the epidemiological features of hantavirus in rodents in Wenzhou,Zhejiang province.Methods Rodents were captured in Wenzhou,where hemorrhagic fever with renal syndrome (HFRS) had been endemic. Hantavirus antigens in the rat lungs were detected by immunofluorescence assay (IFA).Partial S segment (nt 620-999) and partial M segment (nt 2001-2301)sequences were amplified by RT-PCR,and then sequenced.Neighbor-joining method was used to construct for phylogenetic analysis.Results A total of 96 rodents were trapped in the epidemic areas,and 6 hantavirus antigens were identified from these lung samples (6.3%).Partial S and partial M segment sequences were successfully recovered from 5 samples and determined. Phylogenetic analysis of these sequences indicated that all viruses belonged to Seoul virus (SEOV),regardless of the sources (Rattus norvegicus,Rattus tanezumi and Rattus rattoide) that they were derived. However,the clustering pattern in the partial S-tree was different from that in the partial M-tree,suggesting that the re-assortment between SEOVs had occurred.Conclusion All Rattus rats carried SEOV in Wenzhou and the genetic reassortment with SEOV had occurred naturally.
