Establishment of the standard operation program on multiple loci variable numbers tandem repeats analysis typing on Mycobacterium tuberculosis
10.3321/j.issn:0254-6450.2008.09.016
- VernacularTitle:结核分枝杆菌多位点可变数目串联重复序列分型方法标准化操作程序的建立
- Author:
Bing LU
1
;
POURCEL CHRISTINE
;
Jing-Hua LIU
;
Hai-Yan DONG
;
Yuan-Yuan ZHANG
;
Yi JIANG
;
Zhi-Guang LIU
;
Xiu-Qin ZHAO
;
Kang-Lin WAN
Author Information
1. 中国疾病预防控制中心传染病预防控制所
- Keywords:
Mycobacterium tuberculosis;
Multiple loci variable numbers tandem repeats analysis;
Standard operation program
- From:
Chinese Journal of Epidemiology
2008;29(9):919-924
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and evaluate the standardized protocol of multiple loci variable numbers tandem repeats (VNTR) analysis (MLVA) for genotyping Mycobacterium tuberculosis (M.tuberculosis).Methods 15 VNTR loci were chosen for genotyping 54 Chinese M.tuberculosis strains by PCR-electmphoresis-based VNTR analysis and the results were analyzed by software BioNumerics (Version 5.0).Results MLVA method was successfully established and standardized,including the standard protocol for bacterial culture,DNA isolation,PCR and agarose gel electrophoresis and the software analysis. 15 VNTR loci were confirmed,including ETRA,ETRB,ETRC,ETRD,ETRE,MIRU10,MIRU16,MIRU23,MIRU26,MIRU27,MIRU39,MIRU40,Mtub21,Mtub30 and Mtub39,to be suitable for MLVA analysis of M.tuberculosis.Conclusion The standardized MLVA method has been established successfully.This method is simple and has powerful capacity for genotyping and strain differentiation,can be used for the network surveillance on pathogens of M.tuberculosis,and the data are comparable between laboratories.It is valuable for tracing the source and studying the trend of prevalence during investigation of M.tuberculosis infections.
