Epidemiological investigation on natural infection to Borna disease virus (BDV) among horses in Yili,Xinjiang

VernacularTitle:新疆伊犁地区马群中博尔纳病病毒自然感染调查
Author: Dan ZHU ¹ ; Zhi-Lei ZENG ; Dan PENG ; Xiao CHEN ; Li-Bo ZHAO ; Ying-Ying ZHANG ; Ming-Ming XU ; Qun-Ling ZHAN ; Jian-Ping YU ; Peng XIE
Author Information

1. 重庆医科大学附属第一医院
Keywords: Borna disease virus; Nested RT-PCR; TaqMan probe; Horse
From: Chinese Journal of Epidemiology 2008;29(11):1106-1109
CountryChina
Language:Chinese
Abstract: Objective To investigate the epidemiological pattern of Borna disease virus (BDV)infection in horses and to analyze the phylogenetic tree of derived BDV in Yili, Xinjiang. Methods We established a modified nested RT-PCR (nRT-PCR) to detect BDV p24 segment in peripheral blood mononuclear cells (PBMCs) and brain tissues of 120 horses in Yili, Xinjiang. Positive products were analyzed by sequencing and homology analysis. Results The positive rate of BDV infection was 2.5% in both PMBCs and brain tissues at the same time. The gene sequence revealed in positive PCR samples was more than 93 % ,identical to that of BDV derived from horses in other countries. We also noticed a high degree of identity ( >98 % ) to standard strain He/80 in gene sequence of positive PCR samples. Conclusion Our study found the presence of BDV natural infection in horses in Yili. The endemic BDV had a high degree of identity to standard strain He/80.