The emergence of candidate pathogenicity island 89K DNA sequence of Streptococcus suis isolated from sporadic patients in Zhejiang province
10.3321/j.issn:0254-6450.2008.11.013
- VernacularTitle:浙江省散发猪链球菌病临床分离株存在89K候选致病岛
- Author:
Shui-Rong ZHU
1
;
Zhi-Gang WANG
;
En-Fu CHEN
;
Ting-Ting YANG
;
Zheng ZHANG
;
Bao-Xiang XU
;
Da-Zhi JIN
;
Jian-Qin HE
;
Fu-Su WANG
Author Information
1. 浙江省疾病预防控制中心
- Keywords:
Streptococcus suis serotype 2;
Polymerase chain reaction;
89K DNA fragment
- From:
Chinese Journal of Epidemiology
2008;29(11):1110-1113
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the presence of candidate pathogenicity island 89K DNA sequence of Streptococcus suis serotype 2 (SS2) strains isolated from patient in Zhejiang province. Methods Genes and DNA fragments were amplified by PCR, using specific primers, and three amplified fragments of the89K sequence were directly sequenced. The results were analyzed using software related to bioinformaties and epidemiology. Results 8 strains of SS2 all contained 89K sequence, cps2J and mrp virulent genes, and species-specific 16S rDNA. 3 amplified fragments of 89K candidate pathogenicity island of SS2 ZJ0501 were above 99% similar to SS2 strain identified from outbreaks in Jiangsu in 1998, and the gene fragment of coding DNA recombinant protein in the 89K sequence was highly homological with that of S. dysgalactiae and S. agalactiae. Conclusion In recent years SS2 strains isolaed from patients with clinical symptoms in Zhejiang province had been detected to have contained candidate pathogenicy 89K DNA fragment.
