High-performance liquid chromatography-electrospray ionization/tandem mass spectrometry of isofraxidin in Sarcandra glabra.
- Author:
Qi-zhi LUO
1
;
Kai-jin DAI
;
An-de MA
Author Information
- Publication Type:Journal Article
- MeSH: Capsules; Chromatography, High Pressure Liquid; methods; Coumarins; analysis; Drugs, Chinese Herbal; chemistry; Magnoliopsida; chemistry; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization; methods; Tandem Mass Spectrometry; methods
- From: Journal of Southern Medical University 2006;26(12):1821-1823
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for determining isofraxidin concentrations in Sarcandra glabra and Qingrexiaoyanning capsules with high-performance liquid chromatographic-mass spectrometry.
METHODSIsofraxidin was extracted from Sarcandra glabra and Qingrexiaoyanning capsules with acetic ether and chloroform, respectively, and separated by isocratic reversed-phase chromatography. The mass spectrometric system was operated in multiple reaction monitoring mode. A pair of ions: precursor ion m/z 223 with product ion m/z 162 were chosen for the quantification of the analyte.
RESULTSThe retention time of isofraxidin was 6.60 min, and the calibration curve was linear over a concentration range from 484 to 9 680 ng/ml. The average recovery was 96.7% and RSD 4.49%, with detection limit of 1 ng/ml.
CONCLUSIONThe method is rapid, selective and sensitive for determining isofraxidin in Sarcandra glabra and Qingrexiaoyanning capsules.
