Preparation and identification of dengue virus with deletion mutation of capsid protein.
- Author:
Wu-Yang ZHU
1
;
E-de QIN
;
Man YU
;
Cheng-Feng QIN
;
Xue-Dong YU
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Animals; Base Sequence; Capsid Proteins; genetics; Cell Line; Cloning, Molecular; DNA Mutational Analysis; Dengue Virus; genetics; isolation & purification; Electroporation; Molecular Sequence Data; Sequence Deletion; Sequence Homology, Nucleic Acid; Transcription, Genetic; Virus Replication; genetics
- From: Journal of Southern Medical University 2007;27(1):31-37
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo generate rescued viruses with deletion mutation of capsid protein from dengue virus type 2 isolated in China (DEN2-43).
METHODSOn the basis of infectious full-length cDNA clone pD212 of DEN2-43 strain virus, the deletion mutants were constructed by fusion PCR, from which the rescued viruses with deletion mutation of capsid protein were generated by transcription in vitro and electroporation.
RESULT AND CONCLUSIONSequence analysis demonstrated that the deletion mutations had been successfully inserted into the rescued viruses obtained. These mutant viruses may hold the key for elucidating the effects of deletion mutation of capsid protein on the biological characteristics of dengue virus.
