Incorporation and integration of mesenchymal stem cell-derived myocardial cells into host cardiac myocytes: an experimental study in rats.
- Author:
Jun-Ming TANG
1
;
Ming-Jiang WANG
;
Jing YANG
;
Jia-Ning WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; physiology; Cell Proliferation; Connexin 43; analysis; Gap Junctions; physiology; Green Fluorescent Proteins; genetics; metabolism; Immunohistochemistry; Male; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells; cytology; metabolism; physiology; Microscopy, Fluorescence; Myocardial Infarction; pathology; physiopathology; surgery; Random Allocation; Rats; Rats, Wistar
- From: Journal of Southern Medical University 2007;27(1):38-42
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the anatomical bases for electrical coupling of mesenchymal stem cells (MSCs)-derived myocardial cells with host cardiac myocytes.
METHODSMSCs isolated from adult inbred Wistar rats were cultured and expanded in vitro and labeled with gree fluorescence protein (GFP). The model of acute myocardial infarction (AMI) was established in rats of the same species by occlusion of the left anterior descending arteries (LAD). One week after operation, 1.0x10(7) MSCs were injected into the infarcted area and 4 week after transplantation, the cardiac function of the recipient rats was evaluated. The heart tissues were taken after hemodynamic measurement for histological examination and morphometric measurement using image analysis system, and the expression of connexin-43 in the MSCs was detected by immunofluorescent cytochemistry.
RESULTSFour weeks after transplantation, the transplanted MSCs in the infarcted area showed significantly higher expression rate of connexin-43, and the cell transplantation resulted in decreased infarct size and thickening of the left ventricle wall.
CONCLUSIONIn a rat AMI model, MSCs differentiate into myocardial cells which can be integrated into host myocardial cells through formation of gap junction, resulting in effective improvement of the cardiac function.
