Analysis of the difference in proteome expression between yeast form and mould form of Penicillium marneffei using SELDI technique.
- Author:
Dong-Hua LIU
1
;
Xiao-Jun LIU
;
Sheng-Shun TAN
Author Information
- Publication Type:Journal Article
- MeSH: Fungal Proteins; analysis; Penicillium; growth & development; metabolism; Protein Array Analysis; methods; Proteome; analysis; Proteomics; methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; methods; Spores, Fungal; metabolism; Yeasts; metabolism
- From: Journal of Southern Medical University 2007;27(1):59-61
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the difference in protome expression between yeast form and mould form of Penicillium marneffei.
METHODSSurface enhanced laser desorption/ionization (SELDI) time-of-flight mass spectra were performed to compare the expressed proteins between yeast form and mould form of Penicillium marneffei. Protein profiling was read by PBSIIC ProteinChip Reader and the proteome database was analyzed by Proteinchip Software 3.2.0.
RESULTSSeventy-five distinct proteins were found in the yeast form and mould form of Penicillium marneffei, in which 10 proteins were up-regulated in yeast form and 3 in mould form. The proteins 2900 and 3151 were only expressed in the yeast form and the proteins 13,151 and 13,285 only in mould form.
CONCLUSIONSELDI technique can identify the difference of the expressed low-molecular-mass proteins between the mould form and yeast form of Penicillium marneffei.
