Effect of insulin-like growth factor and its receptor-I antibody on growth of human adrenocortical carcinoma SW-13 cell lines in vitro.
- Author:
Wen SHEN
1
;
Jiang XIAN
;
Wei-Lie HU
;
Jun LIU
;
Jia LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adrenocortical Carcinoma; pathology; Antibodies; pharmacology; Cell Line, Tumor; Cell Proliferation; drug effects; Dose-Response Relationship, Drug; Humans; Insulin-Like Growth Factor I; pharmacology; Receptor, IGF Type 1; immunology
- From: Journal of Southern Medical University 2007;27(1):88-91
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of insulin-like growth factor (IGF) and its receptor-I antibody on the growth of human adrenocortical carcinoma SW-13 cell line in vitro.
METHODThe growth curves of SW-13 cell treated with IGF and its receptor-I antibody were obtained by means of MTT assay. The effects of the two agents, added either alone or in combination at different concentrations, on the cell growth were evaluated.
RESULTSIGF significantly promoted proliferation of SW-13 cells, and its effect was positively correlated with its concentrations (P<0.05). IGF receptor-I antibody inhibited the effect of insulin-like growth factor with direct inhibitory effect on proliferation of SW-13 cells (P<0.05).
CONCLUSIONIGF can promote the growth of human adrenocortical carcinoma SW-13 cells via its receptor-I. IGF receptor-I antibody can inhibit the effect of the growth factor, suggesting a possible role of this receptor in the treatment of adrenocortical carcinoma.
